Ua kom cov pa roj carbon monoxide: yog hom tsis-ncov qaumteb qab zib siv ntau dua. Feem ntau, nws yuav tsum tau ntxuav nrog dilute hydrochloric acid, ua raws los ntawm ethanol, thiab tom qab ntawd ntxuav nrog dej. Tom qab ziab ntawm 80 ℃, nws tuaj yeem siv rau kem chromatography. Granular tshuab txais yog qhov kev xaiv zoo tshaj plaws rau kem chromatography. Yog tias nws yog ib qho zoo ntawm cov pa roj carbon kom tsim nyog, nws yog ib qho tsim nyog yuav tsum tau ntxiv cov nyiaj ntawm lub diaomite raws li cov kev siv lim, yog li kom tsis txhob txo qis.
Ua kom cov pa roj carbon ua haujlwm tsis yog-polar adsorbent. Nws adsorption yog rov qab rau Silica Gel thiab Alumina. Nws muaj kev muaj zog muaj zog rau cov khoom tsis muaj-polar. Nws muaj qhov muaj zog tshaj tawm muaj peev xwm hauv cov kua aqueous thiab tsis muaj zog hauv cov kuab tshuaj organic. Yog li, lub peev xwm xaiv tau ntawm dej yog qhov tsis muaj zog thiab cov ocicic hnyav yog muaj zog dua. Thaum cov adsorbed raug kaw los ntawm cov pa roj carbon ntau, cov kuab tshuaj txo qis, thiab cov khoom muaj peev xwm xaiv tsa ntawm Eluent yog txhim kho. Dej cov dej sib xyaw, xws li cov amino acids, suab thaj thiab glycosides, tau cais tawm.